Data sheet
| Assay Range | 9.4- 600 pg/ml |
| Sensitivity | 5 pg/ml |
| Size | 96T |
| Storage | Store at 2 - 8⁰C. Keep Standard and detection antibody at -20⁰C. |
| Expiration | Six months at 2 - 8 ⁰C. |
| Application | For quantitative determination of Human BAMBI. |
| Assay principle | This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). |
| Sample type | Serum, plasma, body fluids, tissue lysates. |
More info
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant BAMBI standard: 3 ng/vial - 2.
2. One 96-well plate precoated with anti-BAMBI antibody.
3. Sample diluent buffer: 12 ml
4. HRP conjugated anti-BAMBI antibody: 1 vial, dilution 1:1000.
5. Detection Antibody diluention buffer: 12ml.
6. Assay diluent buffer: 12 ml.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
BMP and Activin Membrane-Bound Inhibitor Homolog (BAMBI) is a single-pass type I membrane protein that belongs to the BAMBI family. It is a transmembrane glycoprotein related to the transforming growth factor-beta (TGF-beta)-family type I receptors and functions as a negative regulator of TGF-beta signaling during development. BAMBI is highly expression in kidney medulla, placenta and spleen. BAMBI is induced by BMP signaling through the evolutionary conserved BMP-responsive elements in its promoter. BAMBI plays important roles in signal transduction in many developmental and pathological processes.
