Human CT-1 ELISA Kit

Human CT-1 ELISA Kit

FM-E100154

96T

31.2pg/mL-2000pg/mL

Less Than 10 pg/mL

No detectable cross-reactivity with any other cytokine.

Store at 4 for frequent use, at -20 for infrequent use.

Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Two months at 4 and four months at -20.

For quantitative detection of human CT-1 in

cell culture supernates, serum and plasma(heparin, EDTA).

 

1. Lyophilized recombinant human CT-1 standard: 10ng/tube-2.

2. One 96-well plate precoated with anti- human CT-1 antibody.

3. Sample diluent buffer: 30 ml

4. Biotinylated anti- human CT-1 antibody: 130µL, dilution 1:100.

5. Antibody diluent buffer: 12ml.

6. Avidin-Biotin-Peroxidase Complex (ABC): 130µL, dilution 1:100.

7. ABC diluent buffer: 12ml.

8. TMB color developing agent: 10ml.

9. TMB stop solution: 10ml.

 

 

Cardiotrophin-1 (CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), interleukin-6 (IL6), and interleukin-11 (IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80% amino acid identity with the 203-amino acid mouse CT-1 sequence; however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.

 

Human CT-1 ELISA Kit-1X96 Well Plate Image