More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 5.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human TIMP-1 standard: 2 vials
2. One 96-well plate precoated with anti- Human TIMP-1 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
The tissue inhibitor of metalloproteinase 1 (TIMP1), also known as erythroid-potentiating activity (EPA), Fibroblast collagenase inhibitor (Collagenase inhibitor), is a member of the tissue inhibitor of metalloproteinase (TIMP) family in which four proteins TIMP1, TIMP2, TIMP3 and TIMP4 have been identified so far. TIMPs are natural inhibitors of the matrix metalloproteinases (MMPs) which are involved in degradation of the extracellular matrix and exhibit diverse functions in development, tissue remodeling, wound healing and tumor metastasis. TIMPs block access of substrates to the MMP catalytic site by forming 1:1 non-covalent complexes with MMPs. Basically, TIMPs are highly specific for MMPs but may not be able to digest some particular MMP such as MMP14. TIMP-1 is widely expressed by many cells and tissues, and is inducible in response to inflammatory stimuli of IL-1, IL-6, OSM, LIF, TNF-α, TGF-β1 and phorbol esters. In addition to its inhibitory activity on MMPs, TIMP1 also functions as a growth factor that regulates cell differentiation, migration and death and shows erythroid potentiating activity.