Data sheet
| Assay Range | 23.4 - 1500 pg/ml |
More info
Sensitivity
20pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Human CD332 standard: 1.5 ng/vial - 2.
2. One 96-well plate precoated with anti-Human CD332 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD332 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Fibroblast growth factor receptor 2, also known as CD332, is a member of the fibroblast growth factor receptor subfamily belonging to the Tyr protein kinase family, which are related proteins that are involved in important processes such as cell division, regulation of cell growth and maturation, formation of blood vessels, wound healing, and embryonic development. CD332 plays an important role in signaling certain immature cells in the developing embryo to become bone cells in the head, hands, feet, and other tissues. Mutations of CD332 are associated with numerous medical conditions that include abnormal bone development (e.g. craniosynostosis syndromes) and cancer.
