Data sheet
| Assay Range | 47 - 3000 pg/ml |
More info
Sensitivity
40pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Human CD171 standard: 3 ng/vial - 2.
2. One 96-well plate precoated with anti-Human CD171 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD171 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD171, also known as L1CAM, is a transmembrane protein, is a member of the Ig superfamily. It has been shown to function as a cell adhesion molecule mediating homotypic and heterotypic cell-cell interactions in neuronal myelination, neurite outgrowth and regeneration. CD171 involves in cell adhesion molecule with an important role in the development of the nervous system, including neuron-neuron adhesion, neurite fasciculation and outgrowth of neurites. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.
