Data sheet
| Assay Range | 62.5 - 4000 pg/ml |
More info
Sensitivity
50 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Human RELT standard: 2 ng/vial - 2.
2. One 96-well plate precoated with anti-Human RELT Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human RELT Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
RELT (Receptor Expressed in Lymphoid Tissues) is a type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF19-like (TNFRSF19L). The TNFRSF family consists of a group of glycoproteins characterized by cysteine-rich motifs in the extracellular domain (CRDs). RELT is primarily expressed in hematopoietic tissues and peripheral blood leukocytes. This receptor is capable of stimulating T-cell proliferation in the presence of CD3 signaling, which suggests its regulatory role in immune response. It has been shown to activate the NF-kappaB pathway and selectively bind TNF receptor-associated factor 1 (TRAF1).
