Data sheet
| Assay Range | 15.6 - 1000 pg/ml |
More info
Sensitivity
15pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Human ALK-1 standard: 2 ng/vial - 2.
2. One 96-well plate precoated with anti-Human ALK-1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human ALK-1 Ab: 1 vial, dilution 1:400.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Activin A receptor, type II-like1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase1), is a type I cell-surface receptor for the TGF-beta superfamily of ligands including Activin A. ACVRL1 protein is expressed in human placenta, certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Deficiency causes hemorrhagic telangiectasia type 2 also known as Rendu-Osler-Weber syndrome 2.
