Data sheet
| Assay Range | 46.8 - 3000 pg/ml |
More info
Sensitivity
40 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Human SerpinA3 standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-Human SerpinA3 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human SerpinA3 Ab: 1 vial, dilution 1:400.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Serpin A3 is also known as Alpha-1-antichymotrypsin (ACT or AACT), which belongs to the serpin family. Serpin A3 can bind DNA. SerpinA3 is an early-stage acute-phase plasma protein and a serpin that preferentially inactivates chymotrypsin, cathepsin G, and chymase. SerpinA3 can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. It appears to have a multifaceted role and is associated with inflammatory reactions, Alzheimer's disease, malignant melanoma and gastric cancer. Alpha-1-ACT, a serine protease inhibitor, is tightly associated with amyloid plaques in Alzheimer's disease and in normal aged human and monkey brain.
