Data sheet
| Assay Range | 7.8 - 500 pg/ml |
More info
Sensitivity
5 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse CD120 standard: 1 ng/vial - 2.
2. One 96-well plate precoated with anti-Mouse CD120a Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse CD120a Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Tumor necrosis factor receptor superfamily, member 1A (TNFRSF1A), also known as TNFRI, TNF R-p55/p60, and CD120a, is a type I transmembrane protein widely expressed in a variety of cell types. It functions as a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The soluble TNF receptors are truncated forms of cell surface receptors with neutralizing activity on both TNF-α and TNF-β. It has been suggested that the pro-inflammatory and immunosuppressive properties of TNF segregate at the level of its receptors and that the pool of TNF bound to soluble receptors could represent a reservoir to control the TNF release. TNFR1 is also the adapter molecule FADD, which recruits caspase-8 to the activated receptor.
