Data sheet
| Assay Range | 15.6 - 1000 pg/ml |
More info
Sensitivity
15 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human PHPT1 standard:1 ng/vial - 2.
2. One 96-well plate precoated with anti-human PHPT1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human PHPT Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
The 14-kDa phosphohistidine phosphatase (PHP14), also known as PHPT1, was the first histidine phosphatase protein identified in vertebrates and is similar to the janus proteins of Drosophila. PHP14 is a cytoplasm protein, which is highly expressed in HCC tissues and expressed abundantly in heart and skeletal muscle. PHPT1 demonstrates specific phosphatase activity against peptides and proteins containing phosphohistidine. Studies investigating the physiological function of PHP14 have revealed that PHP14 is able to dephosphorylate ATP-citrate lyase and the β-subunit of G proteins in vitro. It has also been suggested that PHP14 plays a role in human lung cancer cell migration and invasion.
