Data sheet
| Assay Range | 78 - 5000 pg/ml |
More info
Sensitivity
70 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD82 standard:5 ng/vial - 2.
2. One 96-well plate precoated with anti-human CD82 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD82 Ab: 1 vial, dilution 1:700.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
The transmembrane glycoprotein CD82, also known as Kangai (KAI)-1,is a membrane glycoprotein that is a member of transmembrane 4 superfamily. CD82 is lymphoid specific and associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. Expression of CD82 has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. The reduced KAI-1 expression may be the result of dysregulated p53 function and could be an important step in the endometrial carcinogenesis.
