Data sheet
| Assay Range | 125 - 8000 pg/ml |
More info
Sensitivity
100 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD44 standard:10 ng/vial - 2.
2. One 96-well plate precoated with anti-human CD44 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD44 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD44, short for Cluster of Differentiation 44, is Single-pass type I membrane protein of the hyaluronate receptor family. As a receptor for hyaluronic acid (HA), CD44 mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is expressed in neuroblastoma cells. Epithelial isoform of CD44 is expressed by cells of epithelium and highly expressed by carcinomas. In cancer cells, CD44 may play an important role in invadopodia formation.
