Data sheet
| Assay Range | 11 - 700 pg/ml |
More info
Sensitivity
10 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human AFP standard:1 ng/vial - 2.
2. One 96-well plate precoated with anti-human AFP Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human AF Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Alpha-fetoprotein (AFP) is a glycoprotein of 591 amino acids and a fetal/tumor-associated protein belongning to the albuminoid gene superfamily. AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. AFP is a marker for certain cancers and congenital defects. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth.
