Data sheet
| Assay Range | 31.2--2000 pg/ml |
More info
Sensitivity
30pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human VSIG4 standard:10 ng/vial - 2.
2. One 96-well plate precoated with anti-human VSIG4 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human VSIG4 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I transmembrane glycoprotein of the B7 family within the Ig superfamily. VSIG4 is abundantly expressed in several fetal tissues. In adult tissues, highest expression in lung and placenta. It is also expressed in resting macrophages. VSIG4 is a phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. VSIG4 is also a potent inhibitor of the alternative complement pathway convertases.
