Data sheet
| Assay Range | 11 - 700 pg/ml |
More info
Sensitivity
10 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human XPNPEP2 standard:1 ng/vial - 2.
2. One 96-well plate precoated with anti-human XPNPEP2 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human XPNPEP2 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
The membrane-bound X-prolyl aminopeptidase (aminopeptidase P) 2, also known as XPNPEP2, belongs to the peptidase M24B family, and has been reported to associate with Aminopeptidase P (APP) activity. XPNPEP2 is expressed in kidney, lung, heart, placenta, liver, small intestine and colon. No expression in brain, skeletal muscle, pancreas, spleen, thymus, prostate, testis and ovary. It can release any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide. XPNPEP2 is a metalloprotease that may play a role in the inflammatory process and other reactions produced in response to injury or infection. XPNPEP2 may also play a role in the metabolism of the vasodilator bradykinin.
