Data sheet
| Assay Range | 3,12 - 200 pg/ml |
More info
Sensitivity
1 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human REG1B standard:1 ng/vial - 2.
2. One 96-well plate precoated with anti-human REG1B Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human REG1B Ab: 1 vial, dilution 1:25.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Lithostathine-1-beta (REG1B) is a type I subclass member of the Reg gene family. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV based on the primary structures of the encoded proteins. REG1B is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. REG1B could also be found in other tissues, which appears either only under pathological conditions, such as Alzheimer's disease (brain), cancer (colon), or during regeneration such as neuronal sprouting in brain and pancreas regeneration.
