Data sheet
| Assay Range | 62.5 - 4000 pg/ml |
More info
Sensitivity
50 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human ICOSLG standard: 4 ng/vial - 2.
2. One 96-well plate precoated with anti-human ICOSLG Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human ICOSLG Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Inducible co-stimulator ligand (ICOSLG), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein which is expressed on activated monocytes and dendritic cells. ICOSLG is a ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory signal for T-cell proliferation and cytokine secretion. It also induces also B-cell proliferation and differentiation into plasma cells. ICOSLG may play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
