More info
Principle
Indirect ELSIA, Antigen coated plate
Detection Range
6.25 - 100 MPL
Size
96T
Specificity 94%
Sensitivity 94%
Assay time
~ 90 min
Shelf Life
12 Months from the Manufacturing Date
Storage
Store at 2 - 8ºC.
Sample volume
10 µL Serum or Plasma
Detection Method
Chromogenic
Kit Components
1. 20 x wash buffer: 1vial
2. One 96-well plate precoated with recombinant antigen
3. Assa diluent buffer: 1 vial
4. HRP conjugated: 1 vial
5. Positive Control: 1 vial
6. Negative Control:1vial
7. Calibrator stock: 1 vial
8. TMB substrate: 1 vial
9. TMB stop solution: 12 ml.
Background
Cardiolipin is an important component of the inner mitochondrial membrane, where it constitutes about 20% of the total lipid composition. In mammalian cells, cardiolipin (CL) is found almost exclusively in the inner mitochondrial membrane where it is essential for the optimal function of numerous enzymes that are involved in mitochondrial energy metabolism. Anti-cardiolipin antibodies (ACA) are antibodies often directed against cardiolipin and found in several diseases, including syphilis, antiphospholipid syndrome, livedoid vasculitis, vertebrobasilar insufficiency, Behçet's syndrome, idiopathic spontaneous abortion, and systemic lupus erythematosus(SLE). They are a form of anti-mitochondrial antibody.
