Data sheet
| Application | Detect autoantibody against Cas9 protein (Streptococcus pyogenes) resulting from gene-editing and targeting process in vivo, for serum/plasma samples from human, mouse, rat, porcine, bovine, dog, monkey, etc. |
| Assay Type | Peroxidase conjugated |
| Assay Time | 90 minutes |
| Sample Type | Serum, plasma |
| Assay Range | Semi-Quantitative Elisa assay - Positive, Negative and Cut-off |
| Sensitivity | 99.5% |
| Specificity | 100% |
More info
CRISPR/Cas9 enzyme has been extensively used in gene editing in both research setting and disease treatment arena. One of the concern is that Cas9 as a foreign and bacterial protein, it may stimulates the host immune system to generate antibodies. The most common subtypes of the antibody are IgM (early but short, appears 1 week after expression or injection and lasts for 2-3 weeks) and IgG ( late but long, appears 2 weeks after expression or injection and lasts several months or years). Novateinbio's Cas9 antibody ELISA is designed to be used for both IgM/IgG detection after Cas9 vector/protein is injected into the host. The antibody detection will be customized based on whichever host is used: human, monkey, mouse, bovine, porcine, etc. This is an indirect ELISA for qualitative antibody detection. A semi-quantitative assay kit can also be created based on the customer's preference.
For more information, please contact techsupport@novateinbio.com