Amphiregulin Human Recombinant ( AREG Human )

Data sheet

Formulation

Lyophilized from a 0.2um filtered concentrated solution in PBS, pH7.4.

Solubility

It is recommended to reconstitute the lyophilized AREG in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by:(a) Analysis by HPLC.(b) Analysis by SDS-PAGE.

Description

Amphiregulin (AREG) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 98 amino acids and having a molecular mass of 11.3 KDa.The AREG is purified by proprietary chromatographic techniques.

Protein Background

Amphiregulin (AREG) belongs to the EGF family of cytokines that contain 10 proteins such as EGF, TGFb, HBEGF, and the various heregulins. These cytokines are synthesized as transmembrane precursors and are categorized by the presence of one or several EGF

Expression host

Escherichia Coli.

Synonyms

Schwannoma-derived growth factor, Colorectum cell-derived growth factor, AR, CRDGF, SDGF, AREGB, MGC13647.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized AREG although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution AREG should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a ca

Amino acid sequence

SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK.

Biological Activity

Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml, corresponding to a specific activity of 100,000-200,000units/mg.