Fibroblast Growth Factor-Basic Bovine Recombinant ( FGF 2 Bovine )

Data sheet

Formulation

The FGF-b Bovine was lyophilized from a concentrated (1mg/ml) sterile solution containing 1% HSA.

Solubility

It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-2 Bovine Recombinant in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

FGF-2 Bovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17250 Dalton. The Fibroblast Growth Factor 2 is purified by proprietary chromatographic techniques.

Protein Background

FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system de

Expression host

Escherichia Coli.

Synonyms

HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Fibroblast Growth Factor 2 Bovine although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b Bovine Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.Pl

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser.

Biological Activity

The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be 6 Units/mg.