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Fibroblast Growth Factor-4 Human Recombinant ( FGF4 Human )
DescriptionFGF4 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids (31-206) and having a molecular mass of 19kDa.The FGF4 is purified by proprietary chromatographic
Data sheet
| Formulation | The FGF4 protein was lyophilized with 2xPBS, pH 7.4. |
| Solubility | It is recommended to reconstitute the lyophilized FGF4 Human Recombinant sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Description | FGF4 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 182 amino acids and having a molecular mass of 19.8kDa. The FGF4 is purified by proprietary chromatographic techniques. |
| Protein Background | FGF4 holds s comprehensive mitogenic and cell survival activities and takes part in a range of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF4 possess oncogenic transforming activity. FGF4 and FGF3, oncogenic growth factors are localized on chromosome 11. Co-amplification of both factors was found in several kinds of human tumors. FGF4 functions in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. |
| Expression host | Escherichia Coli. |
| Synonyms | HBGF4, FGF-4, FGF4, KFGF, HSTF1. |
| Stability | Lyophilized FGF4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-4 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Physical Appearence | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Biological Activity | The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is 0.5ng/ml, corresponding to a specific activity of > 2,000,000units/mg. |