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Fibroblast Growth Factor-basic Human Recombinant, HEK ( FGF 2 Human, HEK )
DescriptionFGF-2 Human Recombinant produced in HEK cells is a non-glycosylated monomer, having a total molecular weight of 17kDa.The FGF-b is purified by proprietary chromatographic techniques.SourceHEK.Physical AppearenceSterile Filtered White
Data sheet
| Formulation | The FGF2 was filtered (0.2um) and lyophilized from 0.68mg/ml in 1xPBS. |
| Solubility | It is recommended to reconstitute the lyophilized FGF-b in sterile PBS containing 0.1% endotoxin-free recombinant HSA. |
| Purity | Greater than 95% as obsereved by SDS-PAGE. |
| Description | FGF-2 Human Recombinant Thermostable produced in HEK cells is a non-glycosylated monomer, containing 154 amino acids and having a total molecular weight of 17kDa.FGF-2 Thermostable is a protein engineered FGF2 in order to enhance its thermostability without modifying its biological function.The FGF-b is purified by proprietary chromatographic techniques. |
| Protein Background | Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. |
| Expression host | HEK. |
| Synonyms | Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b. |
| Stability | Lyophilized FGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Physical Appearence | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Biological Activity | The specific activity was determined by the dose dependent stimulation of proliferation of the Balb/c 3T3 cell line, the ED50 is 0.007ng/ml. |