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Hepatocyte Growth Factor Human Recombinant, HEK ( HGF Human, HEK )
DescriptionHGF Human Recombinant produced in HEK cells is a glycosylated single chain peptide, having a total molecular weight of 70kDa.The HGF is purified by proprietary chromatographic techniques.SourceHEK.Physical AppearenceSterile Filtered White
Data sheet
| Formulation | The HGF was lyophilized from 1mg/ml in 1xPBS. |
| Solubility | It is recommended to reconstitute the lyophilized HGF in sterile PBS containing 0.1% endotoxin-free recombinant HSA. |
| Purity | Greater than 95% as obsereved by SDS-PAGE. |
| Description | HGF Human Recombinant produced in HEK cells is a glycosylated peptide, containing 697 a.a. (Gln-32 to Ser-728) having a total molecular weight of 70kDa.The HGF is purified by proprietary chromatographic techniques. |
| Protein Background | Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. |
| Expression host | HEK. |
| Synonyms | Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF. |
| Stability | Lyophilized HGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence | 32-QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLRVVNGIPT RTNIGWMVSL RYRNKHICGG SLIKESWVLT ARQCFPSRDL KDYEAWLGIH DVHGRGDEKC KQVLNVSQLV YGPEGSDLVL MKLARPAVLD DFVSTIDLPN YGCTIPEKTS CSVYGWGYTG LINYDGLLRV AHLYIMGNEK CSQHHRGKVT LNESEICAGA EKIGSGPCEG DYGGPLVCEQ HKMRMVLGVI VPGRGCAIPN RPGIFVRVAY YAKWIHKIIL TYKVPQS-728 |
| Physical Appearence | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Biological Activity | The specific activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5 and is typically 10-50ng/ml. |