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Leptin Rabbit Recombinant ( Leptin Rabbit )
DescriptionLeptin Rabbit Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16 kDa.The Leptin is purified by proprietary chromatographic
Data sheet
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. |
| Solubility | It is recommended to reconstitute the lyophilized Leptin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 95.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Description | Leptin Rabbit Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16 kDa.The Leptin is purified by proprietary chromatographic techniques. |
| Protein Background | A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores. |
| Expression host | Escherichia Coli. |
| Synonyms | OB Protein, Obesity Protein, OBS, Obesity factor. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg. |
| Biological Activity | Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. |