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Macrophage Colony Stimulating Factor Rat Recombinant ( M CSF Rat )
DescriptionMCSF Rat Recombinant produced in HEK-293 cells is a secreted protein (amino acids Glu33-Arg254). M-CSF is disulfide-linked homodimer containing 2 x 222 a.a chains.SourceHEK293Physical AppearanceSterile Filtered White lyophilized
Data sheet
| Formulation | Lyophilized from a sterile filtered aqueous solution containing 10mM Na3PO4, pH 7.5. |
| Solubility | It is recommended to reconstitute the lyophilized MCSF in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Description | Macrophage Colony Stimulating Factor Rat Recombinant produced in E.coli is a non-glycosylated homodimer, containing 2 x 155 amino acids and having a total molecular mass of 36.2 kDa.MCSF is purified by proprietary chromatographic techniques. |
| Protein Background | Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. MCSF induces cells of the monocyte/macrophage lineage. MCSF plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. |
| Expression host | Escherichia Coli. |
| Synonyms | Macrophage colony-stimulating factor 1, CSF-1, MCSF, Csf1, Csfm. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence | MEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP. |
| Biological Activity | The activity is determined by dose-dependent induction of M-NFS-60 cell proliferation and is typically less than 2 ng/ml. This corresponds to an expected specific activity of 5x105 units/mg. |