Myostatin Human Recombinant, His Tag ( Myostatin Human, His )

Data sheet

Formulation

Filtered (0.4um) and lyophilized from 0.5mg/ml in 0.05M acetate buffer, pH 4.5.

Solubility

Add 0.1M Acetate buffer pH-4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 1

Purity

Greater than 95% as determined by SDS-PAGE.

Description

Total 152AA. M.W. 16.7kDa (calculated). N-terminal His-tag and spacer (43AA highlighted). The AA sequence of the human myostatin part of the fusion protein is corresponding to the UniProtKB/Swiss-Prot entry O14793.

Protein Background

Myostatin (GDF-8) is expressed uniquely in human skeletal muscle as a 12 kDa mature glycoprotein consisting of 113 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor ? superfamily of secreted growth and d

Expression host

Escherichia Coli.

Synonyms

GDF-8, MSTN, Growth/Differentiation Factor 8,MSTN Muscle Hypertrophy.

Reagent Appearance

Filtered white lyophilized (freeze-dried) powder.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDPSSRSAVR SRRDFGLDCD EHSTESRCCR YPLTVDFEAFGWDWIIAPKR YKANYCSGEC EFVFLQKYPH THLVHQANPR GSAGPCCTPT KMSPINMLYF NGKEQIIYGKIPAMVVDRCG CS.