Noggin Human Recombinant, HEK ( Noggin Human, HEK )

Data sheet

Formulation

The Noggin was lyophilized from 1mg/ml in 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized Noggin in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95% as obsereved by SDS-PAGE.

Description

Noggin Human Recombinant produced in HEK cells is a glycosylated homodimer, having a total molecular weight of 65kDa.The Noggin is purified by proprietary chromatographic techniques.

Protein Background

The secreted polypeptide noggin, encoded by the NOG gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matri

Expression host

HEK.

Synonyms

SYM1, SYNS1, NOG.

Stability

Lyophilized Noggin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Noggin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

The specific activity was determined by the dose dependent inhibition of rhBMP4 induced alkaline phosphate production by ATDC5 cells and is typically 20-100ng/ml.