Transforming Growth Factor-Beta 1 Human Recombinant ( TGF b 1 Human Recombinant )

Catalog # PT_42223

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$201.00
DescriptionTGFB1 Human Recombinant produced in Human 293 cells is a homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25kDa. The TGFB1 is purified by proprietary chromatographic
Product Size: 1ug
Quantity

Data sheet

Formulation
Lyophilized from a sterile filtered solution containing 50mM Glycine, 50mM sodium chloride, 10% Trehalose and BSA (4:1 protein to BSA ratio) pH 4.0.
Solubility
It is recommended to reconstitute the lyophilized TGFB1 in sterile 10mM Acetic Acid at a concentration of 0.1 mg/ml, which can then be further diluted to other aqueous solutions.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Description
TGFB1 Human Recombinant produced in Human 293 cells is a glycosylated homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25.6kDa. The TGFB1 is purified by proprietary chromatographic techniques.
Protein Background
Transforming growth factor betas (TGFBetas) mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are ve
Expression host
Human 293 cells.
Synonyms
Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.
Reagent Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Stability
Lyophilized TGFB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB1 Human should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to
Amino acid sequence
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS.
Biological Activity
The ED50 as determined by the dose-dependent inhibition of IL-4-induced proliferation of mouse HT-2 cells is less than 0.05ng/ml, corresponding to a specific activity of 2x107units/mg.
References
1.Title:Imatinib attenuates skeletal muscle dystrophy in mdx mice .Publication:Published online before print March 16, 2009, doi: 10.1096/fj.09-129833 August 2009 The FASEB Journal vol. 23 no. 8 2539-2548 .Link:http://www.fasebj.org/content/23/8/2539.full
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