Proguanylin Human Recombinant ( Proguanylin Human )

Data sheet

Formulation

Sterile filtered and lyophilized from 0.5 mg/ml in deionized H2O.

Solubility

Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

Proguanylin Human Recombinant produced in E. coli, is an 11 kDa protein containing 96 amino acid residues of the human proguanylin and 2 additional amino acid residues (underlined).The Proguanylin is purified by proprietary chromatographic techniques.GPVT

Protein Background

Heat-stable enterotoxins (STa) are small, cysteine-rich peptides secreted by Escherichia coli that are able to induce diarrhea through the stimulation of an intestine-specific receptor-guanylyl cyclase known as STaR. Binding of STa to STaR induces a drama

Expression host

Escherichia Coli.

Synonyms

Guanylin Precursor, Guanylate cyclase activator 2A, Guanylate cyclase-activating protein 1, Gap-IGUCA2, STARA, GUANYLIN.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Purification Method

Three-step procedure using IMAC and size exclusion chromatography before and after refolding.