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Mesencephalic Astrocyte-Derived Neurotrophic Factor Human Recombinant ( MANF Human )
Data sheet
| Formulation | Filtered (0.2um) and lyophilized from a concentrated (1mg/ml) solution in 1PBS, pH7.4. |
| Solubility | It is recommended to reconstitute the lyophilized MANF in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Description | MANF Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18.1 kDa. The MANF is purified by proprietary chromatographic techniques. |
| Protein Background | MANF is a 20kDa protein which belongs to the ARMET family. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress. |
| Expression host | Escherichia Coli. |
| Synonyms | Mesencephalic astrocyte-derived neurotrophic factor, Protein ARMET, ARP, arginine-rich mutated in early stage tumors, Arginine-rich protein. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized MANF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MANF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence | LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL. |
| Biological Activity | The ED50 was determined by its ability to stimulate the proliferation of rat C6 cells is typically 15-25 ug/ml. |
More info
arginine-rich mutated in early stage tumors, Arginine-rich protein, ARP, Protein ARMET, Mesencephalic astrocyte-derived neurotrophic factor