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Beta Nerve Growth Factor Human Recombinant ( b NGF Human )
DescriptionNerve Growth Factor-beta Human Recombinant produced in E.Coli is a non-covalently disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 identical 121 amino acids with a molecular weight of two 13.6 kDa polypeptide
Data sheet
| Formulation | The beta-NGF protein was lyophilized from a 0.2um filtered solution containing 0.1% Trifluoroacetic Acid (TFA). |
| Solubility | It is recommended to reconstitute the lyophilized NGF-b in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Description | Nerve Growth Factor-beta Human Recombinant produced in E.Coli is a non-covalently disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 identical 121 amino acids with a molecular weight of two 13.6 kDa polypeptide monomers.The NGF-b is purified by proprietary chromatographic techniques. |
| Protein Background | NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. |
| Expression host | Escherichia Coli. |
| Synonyms | Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Beta-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NGF-Beta should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence | MSSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA. |
| Biological Activity | The ED50, calculated by its ability to stimulate proliferation of TF-1 cells and is typically 1,000,000units/mg. |
| References | Title: u-Opioid receptor activation in live cellsPublication: Journal 22.10 (2008): 3537-3548.Link: http://www.fasebj.org/content/22/10/3537.full |
| Immunological Activity | Thimerosal and beta propiolactone treatmentThis product has been treated in a manner consistent with methods of inactivation. Generally accepted good laboratory practices appropriate to microbiological/viral safe handling practices and techniques are required at work. |
| Reference | Serological studies of influenza A virus, immunogen for antibody production.Tested with anti-influenza A monoclonal antibodies in ELISA. |