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Human Endothelin-2 ( EDN2 Human )
DescriptionEDN2 contains 21 amino acids having a molecular mass of 2546.97 Dalton.Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.FormulationThe protein (1mg/ml) was lyophilized with no additives.SolubilityIt is
Data sheet
| Formulation | The protein (1mg/ml) was lyophilized with no additives. |
| Solubility | It is recommended to reconstitute the lyophilized EDN2 in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 95.0% as determined by RP-HPLC. |
| Description | EDN2 contains 21 amino acids having a molecular mass of 2546.97 Dalton. |
| Protein Background | Endothelin-2 is a Hypoxia-induced Autocrine Survival Factor for Breast Tumor Cells. The synthesis of EDN2 by human kidney carcinoma cells is decreased by EGF. EDN2 is a chemoattractant for macrophages and THP-1 monocytic cells. Chemotaxis towards EDN2 is via the MAPK pathway: p44 and p42 are phosphorylated when THP-1 cells are stimulated with EDN2. Migration to EDN2 is inhibited by hypoxia and by pertussis toxin. EDN2 leads to activation of macrophages. EDN2 shares a similar peptide sequence with chemokines and may signal via a similar receptor and MAPK-mediated pathway. Furthermore, EDN2 expression by tumors may modulate the behavior of macrophages such that activated cells accumulate in areas of hypoxia. |
| Synonyms | EDN2, EDN-2, vasoactive intestinal contractor peptide, VIC, ET-2, Endothelin-2, Preproendothelin-2, PPET2, ET2. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized EDN2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EDN2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence | Cys-Ser-Cys-Ser-Ser-Trp-Leu-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp. |