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Endothelial Mitogen Bovine ( ECGS Bovine )
DescriptionEndothelial Mitogen can be used for Human & Mammalian Vascular Endothelial Cells at concentration of 0.1-0.3mg/ml of serum supplemented media & 0.01-0.1mg with heparin. Endothelial Mitogen can be used for Hybridoma Cell Cloning at
Data sheet
| Reactivity | Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.013 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Eotaxin as a Reference Standard. |
| Formulation | The Endothelial-Mitogen was lyophilized from a concentrated solution containing 0.1M NaCl and 5mM NaH2PO4, pH-7.4. |
| Solubility | It is recommended to reconstitute the lyophilized Endothelial-Mitogen Bovine Recombinant in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Description | Endothelial Mitogen can be used for Human & Mammalian Vascular Endothelial Cells at concentration of 0.1-0.3mg/ml of serum supplemented media & 0.01-0.1mg with heparin. Endothelial Mitogen can be used for Hybridoma Cell Cloning at concentrations of 0.025-0.15mg/ml of serum supplemented media.Endothelial Mitogen can be used for Balb/C-3T3 Cells at concentrations of 0.2mg/ml of serum Supplemented media. |
| Expression host | Bovine Hypothalamus. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Endothelial-Mitogen Bovine although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Endothelial-Mitogen Bovine Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Caution | The Sale of Eotaxin Human Recombinant by ProSpec is prohibited in the United States. |