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Isocitrate Dehydrogenase-1 Yeast Recombinant ( IDH1 )
DescriptionICDH is isolated from Saccharomyces Cerevisiae.The ICDH is purified by proprietary chromatographic techniques.SourceSaccharomyces Cerevisiae.Physical AppearanceSterile Filtered clear solution.FormulationOne ml of solution (1mg/340ul)
Data sheet
| Formulation | One ml of solution contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1. |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Description | Recombinant Saccharomyces Cerevisiae ICDH (NADP) derived from yeast host cells by using over-expression system, is full length same as designated ICD1 from Saccharomyces Cerevisiae. The N-terminal amino acid Phenylalanine residue next to Met is substituted with Alanine for overexpression. The ICDH is purified by proprietary chromatographic techniques. |
| Protein Background | Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle. |
| Expression host | Yeast cells. |
| Synonyms | Isocitrate dehydrogenase [NADP] cytoplasmic, EC 1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, IDH, NADP(+)-specific ICDH, IDP, PICD. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Unit Definition | One unit is defined as 1 umol of NAD+ production per minute under the assay conditions (25°C, pH 7.5). |
| Biological Activity | The specific activity was found to be 115 U/mg. |