Data sheet
| Assay Range | 234 - 30000 pg/ml |
More info
Sensitivity
200 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Human JAML standard: 30 ng/vial - 2.
2. One 96-well plate precoated with anti-Human JAML Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human JAML Ab: 1 vial, dilution 1:400.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
JAML or Junctional Adhesion Molecule-Like, or AMICA1 is a JAM transmembrane protein family member. The expression is restricted to the hematopoietic tissues with the exception of liver. JAML is also expressed in fetal liver, spleen and thymus by mature leukocytes. A known ligand of JAML is Coxsackie virus and Adenovirus Receptor (CXADR in humans and CAR in mice), which has been shown to localize to the tight junctions of epithelial cells. Upon epithelial CXADR-binding, AMICA1 induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.
