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Mouse EpCAM / TROP-1 / TACSTD1 ELISA Kit

Catalog #: NS-E10245

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Mouse EpCAM / TROP-1 / TACSTD1 ELISA Kit

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Data sheet

Assay Range	78 - 5000 pg/ml

More info

Sensitivity

50 pg/mL

Size

96T

Storage

Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC

Assay Principle

Sandwich ELISA

Sample volume

100 µL final volume, dilution factor varies on samples.

Detection Method

Chromogenic

Kit Components

1. Recombinant Mouse EpCAM standard: 10 ng/vial - 2.

2. One 96-well plate precoated with anti-Mouse EpCAM Ab

3. Sample diluent buffer: 12 mL x 1

4. HRP conjugated anti-Mouse EpCAM Ab: 1 vial, dilution 1:1000.

5. Detection Antibody diluent buffer: 12 mL x 1

6. 20 x Wash Solution: 25 ml x 1.

7. TMB color developing agent A: 6 ml.

8. TMB color developing agent B: 6 ml.

9. TMB stop solution: 6ml.

Background

Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+ independent homotypic cell-cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. EpCAM is expressed on the basolateral membrane of all simple (especially glandular), pseudo-stratified, and transitional epithelia.Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.

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