Data sheet
| Assay Range | 9.4 - 600 pg/ml |
More info
Sensitivity
9 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse CD120b standard: 0.6 ng/vial - 2.
2. One 96-well plate precoated with anti-Mouse CD120b Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse CD120b Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNF exerts its function by binding to and signaling via two different receptors. TNFR1 (CD120a) is constitutively, but in low levels, expressed on nearly all nucleated cell types. TNFR1 activates the caspase family, which induce cell death. TNFR2 (CD120b) is inducible and expressed by cells of myeloid lineage, peripheral T cells and alveolar lymphocytes and macrophagesTNFR2 signalling leads to NF-ĸB transcription and can induce proliferation, differentiation, cytokine production and even apoptosis.
