More info
Assay range | 3.12-200 ng/ml |
Sensitivity | 3.1 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Urine, milk, saliva, and cell culture supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human AT III standard: 1 vial
2. One 96-well plate coated with Human AT III Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human AT III Ab (50x): 140 µL
5. Streptavidin-HRP (100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Antithrombin III (AT III), is a 432 amino acid glycoprotein. It contains three disulfide bonds and a total of four possible glycosylation sites. AT III is a serine protease inhibitor and plays an important role in inactivating several enzymes of the coagulation system, such as thrombin, factor Xa. It also exhibits strong anti-inflammatory activity. AT III deficiency is a rare hereditary disease that predisposes to thrombo-embolic complications. AT III levels are positively correlated with plasma total cholesterol levels, plasma low-density lipoprotein cholesterol levels, plasma triglycerides and D-dimer levels.