More info
Assay range | 0.156-10 ng/ml |
Sensitivity | 0.15 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | plasma, serum, urine, CSF and cell culture supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human SAP standard: 1 vial
2. One 96-well plate coated with Human SAP Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human SAP Ab (50x): 140µL
5. Streptavidin-HRP (100x): 80 µL
6. TMB developing agent: 8 mL x1
7 . Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Serum amyloid P component (SAP) is a member of the pentraxins family, characterized by calcium dependent ligand binding and distinctive flattened β-jellyroll structure similar to that of the legume lectins. SAP is the identical serum form of amyloid P component (AP) which makes up 14% of the dry mass of amyloid deposits and plays an important role in the pathogenesis of Amyloidoses manifested by the ordered aggregation of normal globular proteins and peptides into insoluble fibres which disrupt tissue architecture and are associated with cell death. AP may decorate and stabilize aggregates by preventing proteolytic cleavage and hence inhibiting fibril removal via the normal protein scavenging mechanisms. Human SAP has 51% amino acid sequence homology with C-reactive protein (CRP), a classical acute phase response plasma protein. Serum AP has been implicated in cardiovascular disease, Alzheimer's disease and liver disease.