More info
Assay range | 0.781-200 ng/ml |
Sensitivity | 0.7 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Urine and cell culture supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Rat Album standard: 1 vial
2. One 96-well plate coated with Rat Album Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Rat Album Ab (100x): 80 µL
5. Streptavidin-HRP (100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Serum albumin is the most abundant blood plasma protein, it constitutes about 50% of human plasma protein. Serum albumin plays an important role in regulating blood volume by maintaining the oncotic pressure / colloid osmotic pressure of the blood compartment. It also serves as carriers for molecules of low water solubility including lipid soluble hormones, bile salts, unconjugated bilirubin, free fatty acids (apoprotein), calcium, ions (transferrin), and some drugs. Low albumin (hypoalbuminemia) may be associated with liver disease, nephrotic syndrome, burns, protein-losing enteropathy, malabsorption, malnutrition, while high albumin (hyperalbuminemia) is related to dehydration.