More info
Assay range | 1.56-50 ng/ml |
Sensitivity | 1.5 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | plasma, serum, milk, tissue extract, and cell culture lysates. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human HSP47 standard: 1 vial
2. One 96-well plate coated with Human HSP47 Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human HSP47 Ab (100x): 80µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Heat shock protein of 47 kDa (HSP47), also knwon as serpin H1, clade H, or collagen binding protein 1 is a member of the serpin family of serine proteinase inhibitors. HSP47 is a 418 amino acid molecular chaperone localized at the endoplasmic reticulum lumen (ER) and involved in the collagen folding and secretion. It is shown that HSP47 interacts with collagens I, II, III, IV and V. Its expression is induced by heat shock. Elevated levels of HSP47 and/or autoantibody have been observed in the sera of mixed connective tissue disease patients and other rheumatic autoimmune diseases. HSP47 is also over-expressed in many fibrotic diseases such as systemic sclerosis, pulmonary fibrosis, liver cirrhosis, cicatricial pemphigoid, epidermolysis bullosa acquisita, and keloids. HSP47 might be a potential biomarker or therapeutic target for the treatment of collagen-related fibrosis.