More info
Assay range | 3.12-200 ng/ml |
Sensitivity | 3.0 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sanwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Urine and cell culture supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Rat Hpt standard: 1 vial
2. One 96-well plate coated with Rat Hpt Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Rat Hpt Ab (50x): 140 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Haptoglobin is a 45-48 kDa acute phase glycoprotein belonging to the peptidase S1 family. The full-length human Haptoglobin precursor protein is a 388 amino acid (aa) single-chain polypeptide which can be proteolytically cleaved into disulfide-linked α- and β-chains then dimerizing through α-chain linkages to create a tetramer. Human Haptoglobin shares 80% aa identity with mouse Haptoglobin. Haptoglobin binds to hemoglobin with extremely high-affinity. It detoxifies free hemoglobin dimers in the circulation by burying the oxidative portions and chaperoning hemoglobin to the scavenger receptor CD163 on the surface of monocytes and macrophages for clearance. It is an important marker of hemolysis. High haptoglobin level in plasma may be an indicator of increased cardiovascular risk in obese men, inflammation, atherosclerosis, and systemic sclerosis.