More info
Assay range | 3.12-200 ng/ml |
Sensitivity | 3.0 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Urine or cell supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse FBG standard: 1 vial
2. One 96-well plate coated with Mouse FBG Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Mouse FBG Ab (25x): 280 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Fibrinogen (FBG) is a soluble, large glycoprotein with a molecular weight of 340 kDa. It is composed of two sets of alpha, beta, and gamma polypeptide chains. Fibrinogen is synthesized in the liver by the hepatocytes. During normal blood coagulation, a coagulation cascade activates the zymogen prothrombin by converting it into the serine protease thrombin. Thrombin then converts the soluble fibrinogen into insoluble fibrin strands. These strands are then cross-linked by factor XIII to form a blood clot. It has been shown that Elevated plasma FBG is an independent risk factor for coronary atherosclerosis and ischemic heart disease.