Data sheet
| Assay Range | 4.7 - 300 pg/ml |
More info
Sensitivity
4 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Rat CD86 standard: 1 ng/vial - 2.
2. One 96-well plate precoated with anti-Rat CD86 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Rat CD86 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Cluster of Differentiation 86 (also known as CD86 and B7-2) is an 80 kD protein, which belongs to the immunoglobulin superfamily. It is expressed on antigen-presenting cells and functions as a receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. CD86 may also play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation.
