Data sheet
| Assay Range | 6.25 - 400 pg/ml |
More info
Sensitivity
5 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse CD54 standard:1 ng/vial - 2.
2. One 96-well plate precoated with anti-Mouse CD54 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse CD54 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Mouse ICAM1 (Inter-Cellular Adhesion Molecule 1), also known as CD54, is a type of intercellular adhesion molecule. It is a cell surface glycoprotein which is typically expressed on
endothelial cells and cells of the immune system. It binds to integrin of typeCD11a / CD18,
or CD11b / CD18 and is also exploited by rhinovirus as a receptor. Its expression is activated by p53 in an NF-kappaB-independent manner and induced by TNF alpha in a process that involves IKK beta. ICAM-1 ligation produces pro-inflammatory effects such as inflammatory leukocyte recruitment by signaling through cascades involving a number of kinases, including the kinase p56 lyn.
