Data sheet
| Assay Range | 94 - 6000 pg/ml |
More info
Sensitivity
90 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human BMF standard:6 ng/vial - 2.
2. One 96-well plate precoated with anti-human BMF Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human BMF Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Bcl2 modifying factor (BMF) is a cellular protein, which belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BMF is expressed in B-lymphoid cell and normal B-cells. BMF binds to BCL2 proteins and function as an apoptotic activator, which means it may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified. BCL-2 modifying factor (BMF) is a sentinel considered to register damage at the cytoskeleton and to convey a death signal to B-cell lymphoma 2.
