More info
Assay Range | 281-18,000 pg/mL |
Sensitivity | 280 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human Factor H standard: 1 vial
2. One 96-well plate coated with Human Factor H Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human Factor H Ab (50x): 140 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Complement Factor H is a 1213 amino acid plasma glycoprotein containing 20 tandem complement control protein (CCPs) modules (also known as short consensus repeats) and an alternative spliced 45-kDa polypeptide. Factor H is an essential regulatory component of the alternative pathway of complement. It is critical for prevention of complement activation on host cells and tissues, especially the kidney. It controls the formation and decay of the alternative pathway C3/C5 convertase. It also acts as a cofactor for factor I which proteolytically inactivates C3b when C3b is bound to factor H. Mutations and polymorphisms in Factor H gene have been linked to atypical hemolytic uremic syndrome, membranoproliferative glomerulonephritis, and age-related macular degeneration.