Data sheet
| Storage | 2 - 8 ℃ |
| Assay principle | During the first incubation, the antibodies in the patients’ serum bind to the antigens in the test well. The next incubation allows the enzyme complex to bind to the antigen-antibody complex. After a few washings to remove unbound enzymes, a substrate is added that develops a blue color in the presence of the enzyme complex and peroxide. The stop solution ends the reaction, turning the blue assay color to yellow. |
| INTENDED USE | For the qualitative determination of serum antibodies in humans, primarily IgG, to Trypanosoma cruzi using the ELISA technique. |
| Quality Control | The use of a positive and negative control allows easy validation of kit stability. For a valid test, the positive control must be over 0.50 OD units and the negative control must be under 0.20 units. Should the values fall outside these ranges, the kit should not be used. |
| Satisfaction | 100% |
| Request a quote | sales@novateinbio.com |
| Country of origin | Made Proud in the USA |
More info
Kit Components
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1 |
Micro-late precoated with T. cruzi antigens |
1x96 well |
|
2 |
Negative Control |
1 mL X 1 vial |
|
3 |
Positive Control |
1 mL X 1 vial |
|
4. |
Dilution Buffer |
30 mL x2 |
|
5 |
Wash Solution (20x) |
25 mL x1 |
|
6 |
Anti-human Ig Ab -HRP Conjugate |
11mL x1 |
|
7 |
Chromogen developing Solution (TMB) |
11 ml |
|
8 |
Stop Solution |
11 ml |
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