Data sheet
| Assay Range | 24 - 1500 pg/ml |
More info
Sensitivity
20 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human PTX3 standard:10 ng/vial - 2.
2. One 96-well plate precoated with anti-human PTX3 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human PTX3 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Pentraxin-related protein PTX3, also known as Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor- inducible gene 14 protein, TSG-14 and PTX3, is a secreted protein, which belongs to the pentraxin superfamily. It plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. PTX3 is induced by IL1B/interleukin-1 beta and TNF and produced in several cell types, in particular by mononuclear phagocytes, dendritic cells (DCs), fibroblasts and endothelial cells. PTX3 activates the classical pathway of complement activation and facilitates pathogen recognition by macrophages and DCs.
